PMC

Gas chromatography-mass spectrometry (GC-MS) analysis of products produced in the reactions catalyzed by OOMT1 and OOMT2. Total ion chromatograms are shown. A, A reaction using orcinol as the substrate with OOMT1 as the enzyme. B, A reaction using orcinol as the substrate with OOMT2 as the enzyme. C, A reaction using orcinol monomethyl ether as the substrate with OOMT2 as the enzyme (similar results were obtained with OOMT1). Peak 1, orcinol; peak 2, orcinol monomethyl ether; and peak 3, orcinol dimethyl ether. Mass spectra of the peaks, shown here, matched the mass spectra of the corresponding authentic standards. Reactions were carried out in a total volume of 1 mL with substrate concentration of 2 mm and a total of 1.5 μg of purified protein and were allowed to proceed for 120 min.

Trace of liquid-contact orcinol-ion-selective electrodes in orcinol concentration. The inset shows the calibration curve of the proposed sensors.

FT-IR spectra of the proposed polymers (A) MIP/MAA and (B) MIP/AA. (a) FT-IR for Orcinol, (b) MIP before template removal, (c) MIP after template removal, and (d) NIP.

Basic structures of orcinol paradepsides, orcinol metadepsides, β-orcinol paradepsides, β-orcinol metadepsides, and mixed orcinol β-orcinol depsides.